Our study aims to demonstrate that pathogenic mutations of HNF4A cause dysregulation of several gene expressions and induce hepatic tumorigenesis. Therefore, the immediate gain in knowledge expected from this project is evaluation of the role of the HNF4A mutations in liver cancer onset. To answer this question, we will introduce mutations using CRISPR-Cas9 system in mouse embryos and human normal liver cell lines and subsequently perform functional assays. Our study also aims to support the ongoing efforts at improving the early detection of cancers, thereby contributing to the field of preventive medicine.
Description of the tasks:
Task 1: To test whether Zn finger mutations of HNF4A cause dysregulation of transcriptional networks and induce hepatic tumorigenesis using in vitro and in vivo models.
Task 2: To investigate whether HNF4A ligand domain mutations cause liver cancer development through disrupting their binding specificity to target promoters and their co-factors, which changes the alterations in its transcriptional networks using in vitro and in vivo models.
TYPE of NCN grant:
OPUS – NZ5
Deadline for application:
Form of application:
- Full-time employment
- Selected candidate will receive a salary of 6 000 PLN (monthly average gross, including social security)
- Place of employment:
Institute of Genetics and Animal Breeding of the Polish Academy of Sciences
- Employment starting date: December 2018
- Employment final date: January 2021
- Ph.D. in veterinary, animal science, biotechnology, biology or related discipline;
- Knowledge in the field of veterinary science, molecular and cellular biology;
- Knowledge in the field of gene editing and in vitro cultures;
- Ability to use the following techniques: Western blot, PCR and immunofluorescence staining of proteins in the cell (confocal cell imaging);
- Knowledge and experience in the field of reproduction, breeding and maintaining of mice;
- Ability to work in a multidisciplinary environment (assisting students and supervising their projects together with PI).
- Ability to collect and in vitro culture of mammalian embryos;
- Ability to perform mammalian embryo micromanipulation (pronuclear and cytoplasmic microiniection);
- Ability to prepare tools for micromanipulation of mammalian embryos (holding and microinjection pipette);
- Experience or knowledge with CRISPR-technique is advantageous;
- Ability to communicate fluently in English;
- Ability to communicate well and work in a group;
- Ability to work under the time pressure.
Interested candidates are asked to provide the following documents:
- Motivation letter;
- CV including list of publications, conference presentations, membership in scientific organizations and other awards;
- Ph. D. degree certificate.
- Recommendation letter from a scientific mentor, confirming skills necessary for completing the project;
- Other documents, that in the opinion of the candidate are important when considering him/her for the position;
- Signed statement: I consent to having my personal data processed for the purposes necessary for the recruitment process conducted by the Institute of Genetics and Animal Breeding of the Polish Academy of Sciences.
- Applications in English should be sent to: dr. Hiroaki Taniguchi
Please direct any questions referring to the project directly to the PI of this project:
Dr. Hiroaki Taniguchi
Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, Department of Experimental Embryology
ul. Postępu 36a, 05-552 Jastrzębiec n/Warsaw, mazowieckie, Poland
- The administrator of personal data collected during the recruitment process is the Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, entered into the register of institutes of the Polish Academy of Sciences (kept by the Polish Academy of Sciences) under the number RIN-V-32/98, NIP no. 1230018381, REGON no. 000326196, address: Jastrzębiec, ul. Postępu 36A, 05-552 Magdalenka.
- Personal data provided to the Administrator by the participants of the recruitment process will be processed in accordance with the provisions of the Regulation of the European Parliament and of the Council (EU) 2016/679 of 27 April 2016 on the protection of natural persons with regard to the processing of personal data and on the free movement of such data, and repealing Directive 95/46/EC (General Data Protection Regulation) (OJ.EU.L. of 2016 No. 119/1, hereinafter referred to as "GDPR").
- According to art. 5, 6, 7 and 13 of GDPR, the Administrator informs that:
- Personal data provided by the participant of the recruitment process will be processed on the basis of art. 6 par. 1 letter a) of GDPR, i.e. on the basis of their consent and in the range which is necessary to appropriate realization of PhD studies in English
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